Table 2. Primers used in semiquantitative reverse-transcription (RT)-PCR of xlProminin homologs.
| Targets | Primer pairs | Sequences (5′-3′) | Expected MWs |
|---|---|---|---|
| xlProminin-1 | 1 | GAAATTGGCTTTATCATTGCGGCAGTA | 1939-1993a bps |
| AGTAGCCAATGACTTCATCCATTAACTT | |||
| 2 | AACCTTACCAACCAGATTAGAGGATCA | 1601-1655a bps | |
| ACCTTCCCCTCGATATCAAAGGATG | |||
| xlProminin-2 | 1 | CAGAAGGTCCAAGAGGAGTTTGC | 1800 bps |
| CCAGGGATTAGTTATATTGTCACACA | |||
| 2 | GTGCTTGAAAAGATTGTATCTTCTTTC | 1370 bps | |
| AAGGCATTGGCTTCACTCTGTAGC | |||
| xlProminin-3 | 1 | GAAGTTGACTTAATAATTGGCCAAAGT | 1881 or 1902b bps |
| GAGAGCAATGAGAGAGACATTTCAG | |||
| 2 | CATGTTGGCTGGAAATATCTGCATGTT | 1436 bps | |
| AACTGTCTTTCGCCAGCGTAGC | |||
| X. laevis β-actin (as control) | GGCTATGCTCTACCACATGCCA | 532 bps | |
| TGGAGCCACCAATCCAGACA |
aThe expected MWs of these fragments varies with the presence or absence of the alternatively spliced exons 8a (24 bps), 11a (12 bps) and 19 (18 bps). The differences in sizes of these PCR products are too small to be resolved on a 1% agarose gel.
bThe expected MWs of these fragments varies with the presence or absence of the alternatively spliced exons 24a (21 bps). The differences in sizes of the two PCR products are too small to be resolved on a 1% agarose gel.