Table 1.

Sensitivity of the used sample preparations compared to the gold standard for identifying T. brucei s.l. by PCR (n = 300)

Material	True positive	False positive	True negative	False negative	% NPV (95%CI)	% Sensitivity (95%CI)	Kappa value
Whole blood on FTA®cards (10 separate discs)	36	0	199	65	75.4% (70.2-80.6)	35.6% (26.3-45)	0.4
Whole blood on FTA®cards (elution)	57	0	199	44	81.9% (77.1-86.7)	56.4% (46.8-66.1)	0.6
DNA extract	69	0	199	32	86.1% (81.7-90.6)	68.3% (59.2-77.4)	0.7
Lysed blood on FTA®cards (elution)	74	0	199	27	88.1% (83.8-92.3)	73.3% (64.6-81.9)	0.8
Lysed blood on FTA®cards (10 separate discs)	74	0	199	27	88.1% (83.8-92.3)	73.3% (64.6-81.9)	0.8