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Characterization of EPCs and ECs. qPCR of (A) VEGFR2, (B) VE-cadherin and (C) CD31 expression at increasing confluency, relative to 60% confluent ECs (mean ± SEM; n = 3). Columns with different letters are significantly different (P < 0.05). ICC of VEGFR2, CD133 and CD34 in (D) EPCs and (E) ECs. Scale bar = 30 μm. (F) Uptake of DiI-ac-LDL (red) and staining with lectin (green). Nuclear staining (blue) with DAPI-containing mounting medium.
