Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
the sequences of primers.
| Target gene | Primers | Annealing temperature (°C) |
|---|---|---|
| Fas | Forward:5'-AGCTTGGTCTAGAGTGAAAA-3' Reverse: 5'-GAGGCAGAATCATGAGATAT-3' |
51 |
| FasL | Forward: 5'-CACTTTGGGATTCTTTCCAT-3' Reverse: 5'-GTGAGTTGAGGAGCTACAGA-3' |
57 |
| GAPDH | Forward: 5'-GAAGGTGAAGGTCGGATGC-3' Reverse: 5'-GAAGATGGTGATGGGATTTC-3' |
61 |
