Table 2.
The particle size and zeta potential of FA-PEAs:VHLpolyplexes
| Weight Ratio | Particle size (nm) (±SD) | Zeta potential (mV) (±SD) | ||
|---|---|---|---|---|
| (Nanoparticles versus VHL plasmids a) | FA-PEAs: VHL | PEI(2-kD): VHLc | FA-PEAs: VHL | PEI(2-kD): VHLc |
| 5:1 b | 277.5 ± 10.1 | 304.1 ± 5.7 | 4.4 ± 0.4 | 5.1 ± 0.3 |
| 10:1 | 238.8 ± 18.8 | 289.1 ± 8.9 | 7.1 ± 0.6 | 5.9 ± 0.3 |
| 15:1 | 182.4 ± 6.5 | 250.4 ± 4.6 | 11.4 ± 1.1 | 9.1 ± 0.2 |
| 20:1 | 165.4 ± 11.6 | 223.1 ± 12.5 | 15.6 ± 0.7 | 12.4 ± 0.9 |
| 25:1 | 143.5 ± 11.4 | 222.5 ± 9.3 | 22.4 ± 2.6 | 18.4 ± 0.5 |
| 30:1 | 140.3 ± 1.5 | 157.4 ± 5.1 | 31.6 ± 0.8 | 22.5 ± 0.7 |
SD stands for standard deviation. All data were measured three times (n = 3).
(a) The VHL plasmids were constant (2 μg), and the total volume of complexes was 1 ml.
(b) The concentration of free FA-PEAs was 10 μg/ml.
(c) The PEI (2-kD):VHL complexes were used as a control.