Figure 3. Yeast cells lacking BXI1 are more susceptible to ethanol-induced and glucose-induced programmed cell death.

Wildtype and Δbxi1 cells from the BY4742 strain background were cultured in rich YPD media overnight and resuspended in fresh media and allowed to reach exponential phase (an approximate OD₆₀₀ value of 0.4). (A) Exponential growing yeast cells were then resuspended in fresh media or in fresh media containing 22% ethanol, allowed to grow at 30°C for the indicated times, serially diluted onto YPD plates, and cultured at 30°C for 2 days. (B) Exponential growing yeast cells were then resuspended in water or in 2% glucose, allowed to grow at 30°C for the indicated times, serially diluted onto YPD plates, and cultured at 30°C for 2 days. The differences in viabilities were deemed statistically significant by the Student's t-test (p<0.05). Error bars indicate standard deviations for trials with at least three independent cultures.