Figure 7. Deleting BXI1 diminishes calcium signaling in the absence and presence of unfolded proteins in the ER.

Cells of the indicated genotype in the Σ1278b strain background were transformed with a 4XCDRE-lacZ reporter, grown overnight in selective media at 30°C. They were then resuspended in fresh media and allowed to reach exponential phase (an approximate OD₆₀₀ value of 0.4). Next, they were resuspended in fresh media or in fresh media containing 0.6 µg/ml TM and allowed to grow at 30°C for three hours. β-galactosidase assays with the colorimetric substrate o-nitrophenyl-β-D-galactopyranoside (ONPG) were then performed and quantified using the permeabilized cell method as previously described. [37], [41], [42] The differences in β-galactosidase levels were deemed statistically significant by the Student's t-test (p<0.05). Error bars indicate standard deviations for trials with at least three independent transformants.