Table 2. Effect of L-NNA, ODQ and potassium channel blockers on ACh-induced relaxation of mesenteric arteries from normal and diabetic rats with or without 3′, 4′-dihydroxyflavonol (DiOHF, 1 mg/kg s.c. daily for 7 days) treatment in the presence of indomethacin.

		Normal			Normal + DiOHF			Diabetic			Diabetic + DiOHF
	n	pEC50	Rmax (%)	n	pEC50	Rmax (%)	n	pEC50	Rmax (%)	n	pEC50	Rmax (%)
Control	12	7.94±0.13	100±0	10	7.78±0.16	100±0	11	6.86±0.12†	97±3	11	7.49±0.13§	100±0
TRAM-34 + apamin	12	7.26±0.19*	91±3	9	6.93±0.22*	92±2	11	6.86±0.16	66±8* †	11	7.04±0.11*	86±3* §
TRAM-34 + apamin+Ibtx	10	6.83±0.15*	68±10*	9	6.84±0.19*	71±11*	10	ND	31±9* †	11	6.98±0.12*	69±6* §
30 mM K+	8	6.91±0.10*	78±4*	9	7.28±0.11* †	73±4*	8	6.35±0.07* †	52±5* †	7	6.81±0.13* ‡ §	70±2* §
L-NNA + ODQ	12	7.14±0.12*	100±0	10	7.07±0.15*	98±1	10	6.63±0.15†	97±1	11	6.85±0.12*	99±1
L-NNA + ODQ + TRAM-34 + apamin	10	5.48±0.23*	59±10*	10	6.10±0.30*	70±9*	6	ND	1±1* †	7	ND	2±2* ‡
L-NNA + ODQ + TRAM-34 + apamin+Ibtx	7	ND	3±3*	5	ND	2±2*

A comparison of the sensitivity (pEC₅₀) and maximum relaxation (R_max) to ACh in the absence (control), or in the presence of TRAM-34 (1 µM)+apamin (1 µM), TRAM-34 (1 µM)+apamin (1 µM) +Ibtx (100 nM), 30 mM K⁺, L-NNA (100 µM)+ODQ (10 µM), L-NNA (100 µM)+ODQ (10 µM)+TRAM-34 (1 µM)+apamin (1 µM) or L-NNA (100 µM)+ODQ (1 µM)+TRAM-34 (1 µM)+apamin (1 µM)+Ibtx (100 nM) in endothelium intact mesenteric arteries. All experiments were conducted in the presence of indomethacin (10 µM). n = the number of experiments.

*Significantly different to control within each group, p<0.05, Dunnet's test,

^†Significantly different to normal within inhibitor group, p<0.05, Bonferroni's test.

^‡Significantly different to normal+DiOHF within inhibitor group, p<0.05, Bonferroni's test.

^§Significantly different to diabetic within inhibitor group, p<0.05, Bonferroni's test. Results are shown as mean±SEM, ND =  Not determined.