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. 2011 Jun 6;6(6):e20848. doi: 10.1371/journal.pone.0020848

Figure 2. Effect of constitutively active and dominant negative AMPK mutants on NFκB activation.

Figure 2

Human endothelial cells infected with either control (CTL) virus or adenoviral constructs encoding (A) constitutively active (CA) or (B&C) dominant negative (DN) AMPK mutants. After 48 hours cells were treated with TNF-α (1 or 10 ng/mL) for 30 minutes and NFκB activity determined by EMSA. In some experiments (C), cells were pre-treated with DETA-NO (100 µmol/L) prior to TNF-α stimulation. The bar graph summarizes the results of data obtained in 4 (A), 6 (B) or 5 (C) independent experiments; *P<0.05, **P<0.01, ***P<0.001 versus the appropriate Sol group and §P<0.05, §§P<0.01, §§§P<0.001 versus cells treated with CTL virus.