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. 2011 Jun 6;6(6):e20965. doi: 10.1371/journal.pone.0020965

Figure 2. STAT3 decoy ODN increased the drug sensitivity via inhibiting the transcription of mdr1 and increasing the accumulation of adriamycin.

Figure 2

(A) Luciferase reporter assay and western blot were used to detect the STAT3 activation and expression after inhibiting by decoy ODN. K562/A02 cells were co-transfected with STAT3 or STAT6-Luciferase reporter construct and decoy/scrambled ODN, and then luciferase activity was measured. Total STAT3, pY705 STAT3 were detected by western blotting. (B) STAT3 decoy ODN increased the sensitivity of K562/A02 cells to adriamycin. K562/A02 cells were transfected with 200nM STAT3 decoy or scrambled ODN. After 6h, different concentrations of adriamycin were added and incubated for 48h followed by the CCK-8 assay. (C) STAT3 decoy ODN inhibited the transcription and expression of mdr1. K562/A02 cells were transfected with STAT3 decoy or scrambled ODN, and mRNA and protein levels of mdr1 were measured by real time-PCR and western blotting. (D) STAT3 decoy ODN increased the accumulation of adriamycin and rhodamine 123 into K562/A02 cells. K562/A02 and K562 cells were pretreated with the STAT3 decoy or scrambled ODN, and then treated with adriamycin or rhodamine 123. Fluorescence intensity of adriamycin and rhodamine 123 were determined by flow cytometry.