Figure 5. Sucrose gradient polysome analysis of TbRACK1 expressed in L. major.

LACK/TbRACK1 L. major lysates were prepared, fractionated and blotted as described in Fig. 2. Blots were probed with anti-LACK, anti-TcPO and anti- eEF1A antisera as indicated. Fractions are numbered relative to the top and bottom of the sucrose gradient, as indicated.