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. 2011 Jun 3;6(6):e20710. doi: 10.1371/journal.pone.0020710

Figure 6. Co-immunoprecipitation analyses of potential interactions between LACK and TbRACK1, and eEF1A.

Figure 6

L. major lines expressing either an HA-epitope-tagged form of LACK or TbRACK1 were created and analysed as depicted for LACK/TbRACK1 in Figure 3. Lysates from these two lines were analysed by co-immunoprecipitation under conditions previously described [18], using either anti HA-antibody coupled to agarose beads or anti-TbRACK1 antisera incubated with protein A/G agarose in panels A and B, respectively. Total lysate inputs are indicated. Note that for the total lysate input in Figure 6B, the immunoprecipitating antibody has also been added to the lysate. Arrow indicates L. major eEF1A.