Table 1. Cardiac functional analysis in the progenies obtained from cross-breeding between K51R-Tg and Nkx2.5^+/− mouse lines.

Genotypes
	wt	Nkx2.5+/−	K51R-Tg	K51R-Tg/Nkx2.5+/−
	(n = 3)	(n = 4)	(n = 3)	(n = 4)
Diameters; s (mm)	2.01±0.47	1.91±0.37	1.97±0.05	2.85±0.35
Diameters; d (mm)	3.36±0.36	2.94±0.28	3.43±0.04	3.84±0.28
Volume; s (µl)	15.50±9.08	13.78±5.67	12.32±0.83	33.08±8.42
Volume; d (µl)	47.75±12.68	34.50±7.25	48.47±1.38	64.95±10.93
stroke volume (µl)	32.25±3.95	21.22±3.01	36.14±2.17	31.88±3.84
%EF	72.32±9.33	65.97±9.59	74.45±2.37	51.68±7.53
%FS	41.75±7.26	36.84±7.13	42.45±2.14	26.59±4.96
cardiac output (ml/min)	12.87±2.24	9.39±1.39	14.41±0.41	13.42±1.83
LV mass (mg)	123.17±12.72	111.88±18.32	113.53±11.84	128.80±16.49
BW (g)	25.25±2.80	25.25±2.33	25.73±1.95	24.65±2.95
LV mass/BW	4.60±0.42	4.60±0.93	4.40±0.16	5.30±0.51

Echocardiography was performed on animals of 4 months age as described in Materials and Methods. Note that although there was no statistically significant difference reached in all parameters shown between compound K51R-Tg/Nkx2.5^+/− group and the other three groups due to small size of the sample examined, there was a trend of increase in diastolic and systolic volumes, and a trend of decrease in cardiac function (%EF and %FS) in the compound K51R-Tg/Nkx2.5^+/− mice compared with the other group animals. EF, ejection fraction; FS, fractional shortening.