Figure 5. BMP-4 treatment of sorted P1 population improves their survival in adult mouse striatum.

Freshly sorted cells P1 cells were resuspended in medium supplemented with 40 ng/ml of BMP-4 at a density of 5×10⁴ cells/µl (2 µl /animal) and injected into the right striatum of intact C57-BL6 mice and their survival was analysed 4 weeks later. (A–D): Representative micrographs from BMP4 group show that some of the implanted immature neurons survived and expressed the mature neuronal marker; NeuN. (E): Confocal micrograph from the same group shows some GFP-NeuN immunoreactive cells. (F): Bar graph shows the percentage of survived Tau-GFP and NeuN-IR cells in control and BMP4 groups as of total cells (1×10⁵ cells/animal) transplanted (mean±SEM; n = 4 animals/group, * P<0.05, ***P = 0.0002, Student's t-test). Scale bars = 20 µm. Abbreviation: NeuN = Neuronal nuclei.