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FACS analysis of HTM cells for DEX-induced changes in the expression and activation state of αvβ3 integrins. HTM cells were treated with ethanol (−DEX) or 500 nM DEX for 4 days before FACS analysis. Cells were lifted nonenzymatically and were labeled with nonspecific mouse IgG (solid gray peaks) or with either anti-αvβ3 or anti-activated β3 integrins (open peaks). Primary antibodies were detected with Alexa 488-conjugated anti-mouse IgG.
