Figure 1.

NKT-cell–deficient mice developed fewer liver metastases arising from intraocular tumor and intrasplenic tumor injection. (A) Liver metastases in mice harboring intraocular B16LS9 melanomas. (B) Liver metastases in wild-type mice treated by either splenectomy or low-dose cyclophosphamide before injecting melanoma cells into the VC. (C) Liver metastases after intrasplenic injection of B16LS9 melanoma cells. **P < 0.01. All three experiments were performed three times with similar results. n = 10 mice/each group. Results are expressed as the mean ± SD.