Figure 3.

(A) The linkage map showing the localization of the platinum mutation to the region surrounding vps11 on chromosome 10. Recombination frequencies are shown in parentheses. (B) The platinum mutant zebrafish contained a single-base change and a single-base insertion at nucleotide 1894 in the vps11 open reading frame. (C) Protein sequence comparison of human VPS11, wild-type zebrafish (D. rerio) Vps11 and the deduced amino acid sequence of the Vps11 protein in platinum mutants, showing identical (black) and conserved (gray) residues. The Vps11 protein in platinum mutant zebrafish contains the conserved clathrin repeat domain (red underscore), but not the RING-H2 domain (green underscore).