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. 2011 May 11;52(6):3119–3128. doi: 10.1167/iovs.10-5957

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Figure 4. — (A) Normal pigmentation in an noninjected embryo at 48 hpf. Inset: darkly pigmented melanophores in the trunk of the embryo. (B) An 48 hpf embryo injected with the vps11 morpholino (vps11 MO). Inset: reduced pigmentation in the vps11 morphant melanophores. (C) The dorsal head region of an noninjected embryo at 48 hpf. (D) The dorsal head region of a vps11 morphant at 48 hpf containing melanophores with reduced pigmentation. (E) Alteration of vps11 mRNA splicing in vps11 morphants. Top: normal splicing of β-actin in both noninjected (lane 2) and vps11 morphants (lane 4). Bottom: inhibited splice-removal of the second intron of the vps11 transcript in vps11 morphants (lane 4, double arrowheads).