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. 2011 May 11;52(6):3119–3128. doi: 10.1167/iovs.10-5957

Figure 8.

Figure 8.

(A) Histologic section from a wild-type retina and a (B) platinum mutant retina at 7 dpf. (C) The margin of the wild-type retina shown in (A), showing the interdigitation of the photoreceptors and RPE (arrowheads). (D) Immunolocalization of rhodopsin expression (Rho) in the rod outer segments of wild-type rod photoreceptors at 7 dpf. (E) The margin of the platinum retina shown in (B), showing RPE degeneration and loss of the photoreceptor/RPE boundary. (F) Immunolocalization of Rho in a platinum mutant retina at 7 dpf, showing truncated and collapsed ROS (arrowhead). (G) Histologic section of a wild-type retina showing the elongated RPE nuclei (arrows) and interdigitation of the RPE with the underlying photoreceptors. (H) TEM of wild-type retina at 7 dpf, showing healthy rod outer segments and RPE with melanosomes. (I) Histologic section of a platinum mutant retina at 7 dpf. The neural retina has detached from the RPE. Note the presence of vacuoles (white arrowheads) in the RPE and pyknotic nuclei in both the RPE (white arrow) and photoreceptor layer (black arrowheads). (J) TEM of a platinum mutant retina at 7 dpf. Note the degeneration of the rod outer segments and large vacuoles full of discs from rod outer segments (arrowhead). (K) Glutamine synthetase (GS) localization in wild-type retina at 7 dpf showing Müller glial cells (arrow). (L) Immunolocalization of GS in a platinum mutant retina at 7 dpf, showing Müller glial cell hypertrophy. (M) Immunolocalization of PCNA-positive retinal progenitors in the CMZ of a wild-type retina at 7 dpf. (N) PCNA immunolocalization reveals abnormal proliferation in a platinum mutant retina at 7 dpf. PCNA-positive nuclei are present in all three nuclear layers and in the CMZ (arrowhead). (O) TEM of a wild-type retina, showing elongated and fully pigmented melanosomes in the RPE. (P) TEM of a platinum retina, showing reduced pigmentation in melanosomes and a degeneration of the RPE. (Q) Close-up of a region in (O) showing wild-type melanosomes in three (of the four) stages of maturation, including fully mature melanosomes completely full of pigment. (R) Close-up of the platinum mutant RPE shown in (P). The melanosomes observed in platinum mutants are in an immature phase of melanosome development (melanosomes in stage 3 are shown). Scale bar: (A–F, K, L) 50 μm; (G, I) 20 μm; (M, N) 15 μm; (O, P) 2 μm; (Q, R) 1 μm.