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. 2011 May 13;52(6):3187–3192. doi: 10.1167/iovs.10-6558

Figure 5.

Figure 5.

Effect of enzyme or chemical pretreatment on the killing ability of VH. (A) Growth change (−Δlog CFU/mL) of RN6390ΔtarO after a 24-hour incubation in VH; heated VH and VH treated with protease K are shown. Protease K treatment diminished the VH killing effect. (B) Growth change (−Δlog CFU/mL) of RN6390ΔtarO after a 24-hour incubation in VH treated with 1% DMSO or a protease inhibitor (PI) cocktail are shown. PI-treated VH lost its ability to kill RN6390ΔtarO. (C) Growth change (−Δlog CFU/mL) of RN6390ΔtarO after a 24-hour incubation in VH treated with 1% DMSO or protease inhibitors is shown. Only VH treated with the serine protease AEBSF lost its ability to kill RN6390ΔtarO. Data are expressed as the mean ± SEM (n = 3).