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. 2011 May 16;52(6):3200–3206. doi: 10.1167/iovs.10-6485

Figure 1.

Figure 1.

Hyperosmotic stress-induced c-Jun phosphorylation and Plk3 activation. (A) Effect of high sorbitol concentrations on c-Jun phosphorylation after a time course. (B) Extracellular sorbitol concentration-dependent increases in phosphorylation of c-Jun. (C) Hyperosmotic stress-induced c-Jun activation in HCE, PHCE, and HTCE cells. (D) Hyperosmotic stress-induced increases in Plk3 activity. Hyperosmotic stress-induced Plk3 activation was determined by immunocomplex kinase assay, and c-Jun fusion protein was used as the substrate. (E) Effect of increased concentrations of various extracellular solutes on c-Jun phosphorylation detected by Western blot analysis. All experiments were repeated four times. *P < 0.05.