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. 2011 May 16;52(6):3200–3206. doi: 10.1167/iovs.10-6485

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Figure 2. — Effect of hyperosmotic stress-induced activation of signaling pathways. (A) Effect of hyperosmotic stress generated by various extracellular solutes on Plk3 activation. Plk3 activity was determined by immunocomplex kinase assay. (B) Effect of hyperosmotic stress on JNK activity. (C) Effect of hyperosmotic stress on p38 activity. (D) Determination of site-specific phosphorylation of c-Jun catalyzed by activated Plk3 in a sorbitol concentration-dependent manner. (E) Time course of hyperosmotic stress-activated Plk3 to phosphorylate c-Jun at sites of S63 and S73. (F) Determination of site-specific phosphorylation of c-Jun catalyzed by activated Plk3 in cells induced by high concentrations of different extracellular solutes. Hyperosmotic stress was generated by extracellular sorbitol (300 mM), glucose (300 mM), sucrose (300 mM), and NaCl (300 mM), respectively.