Skip to main content
. 2011 May 16;52(6):3200–3206. doi: 10.1167/iovs.10-6485

Figure 5.

Figure 5.

Effect of Plk3 activation on hyperosmotic stress-induced cell death. (A) Effect of different sorbitol concentrations on survival cell index. (B) Effect of inhibiting JNK with SP600125 on hyperosmotic sorbitol-induced decreases in survival cell index. (C) Effect of overexpression of wild-type Plk3 and active Plk3 mutants on survival cell index in hyperosmotic stress-induced cells. (D) Comparison of hyperosmotic stress- and UV irradiation-induced apoptosis. (E). Effect of overexpressing wild-type Plk3 on hyperosmotic stress-induced apoptosis at 1 hour and 2 hours. (F) Effect of knocking down Plk3 mRNA on hyperosmotic stress-induced apoptosis. (G) Effect of inhibiting JNK with SP600125 on hyperosmotic sorbitol-induced apoptosis. (H) Hyperosmotic stress-induced increases of annexin V level in Plk3-wt and Plk3K52R transfected cells. Survival cell index and apoptosis were evaluated by MTT assays and by measuring caspase 3 activity and annexin V levels, respectively. Significant differences in data collected from four independent experiments (*P < 0.05; n = 4).