Figure 9.
Effect of inhibition of P2X3 receptors on ATP-stimulated [Ca2+]i and protein secretion. Acini containing fura-2 were preincubated with either A317419 (10−6–10−4 M, A) for 30 minutes before stimulation with ATP (10−6 M). Peak [Ca2+]i was calculated from three animals. Data are mean ± SEM. Acini were also preincubated with A317491 (10−5 M) for 30 minutes and stimulated with ATP (10−6–10−4 M) for 40 minutes. Peroxidase secretion was measured (B). Data are mean ± SEM from four animals. ⧣Significant difference from ATP alone. *Significant difference from no additions. ATP alone data are repeated from Figure 3C because these two assays were performed at the same time.