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. 2011 May 20;52(6):3368–3380. doi: 10.1167/iovs.10-6991

Figure 7.

Figure 7.

LY294002 and PP2 sensitizes ARPE-19 cells to Nutlin-3–induced apoptosis. (A) Serum-starved ARPE-19 cell monolayers were pretreated with or without Src kinase inhibitor (PP2; 10 μM) and PI3-kinase inhibitor (LY294002; 10 μM) for 1 hour, followed by treatment with 60 μM Nutlin-3 for 3 hours. An equal volume of DMSO was used as a control. Samples were analyzed for DNA fragmentation using ELISA (mean ± SEM; n = 3). *P < 0.05; significantly different compared with Nutlin-3. (B) Western blot analysis for the levels of phospho- and total- p53, Mdm2, Akt, and Bcl-2 are shown. Actin was used as a loading control.