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. 2011 May 20;52(6):3368–3380. doi: 10.1167/iovs.10-6991

Figure 9.

Figure 9.

Knockdown of p53 by gene-specific siRNA inhibits Nutlin-3–induced apoptosis. (A) ARPE-19 cells were transfected with control or p53-specific siRNA. Cell lysates were analyzed by Western blot for the levels of p53, p73, p21Cip1, Bcl-2, and Bcl-xL using specific antibodies. (B) Cells transfected with control or p53 siRNA were treated with 20 μM CPT for 3 hours or with 60 μM Nutlin-3 for 2 hours. Cell extracts were analyzed to determine the levels of PUMA, Siva-1, active caspase-3, and actin by Western blot analysis.