Figure 9.
Effect of cholinergic and P2X7 receptor antagonists on protein secretion stimulated by carbachol or BzATP from lacrimal gland acini. Peroxidase secretion, our index of protein secretion, was measured from lacrimal gland acini preincubated with the P2X7 receptor antagonist A438079 (10−4 M) for 30 minutes and then stimulated with carbachol (Cch) (10−4 M) for 40 minutes (A) or preincubated with the cholinergic antagonist atropine (10−4 M) for 30 minutes and then stimulated with BzATP (10−4 M) for 40 minutes (B). Values are mean ± SEM from four independent experiments. *Significant difference from no additions. #Significant difference from agonist.