Figure 4.

Early-onset, slow progression of cone degeneration in CNGB3^−/− mice evaluated by Western blot detection of expression of cone-specific proteins. Western blot detection was performed using retinal membrane protein extracts prepared from CNGB3^−/− and WT mice at 1 and 9 months to determine the expression of M-opsin, Gnat2, and CAR. Actin was included as a loading control. Shown are the representative images (A) of these blots and the corresponding quantification (B). Data represent mean ± SD (n = 4–7 mice for each group). Unpaired Student's t-test was used to determine significance between age-matched CNGB3^−/− and WT mice (*P < 0.05).