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Immunoprecipitation of Hsp60 and MMP2. BRECs, incubated in 5 mM or 20 mM glucose for 4 days, were immunoprecipitated with anti-Hsp60 or anti-MMP2. Relative abundance of MMP2 in the Hsp60 immunoprecipitates (A) and that of connexin43 in MMP2 immunoprecipitates (B) were determined by Western blot analysis using IgG as the loading control. Results are expressed as mean ± SD of three or more experiments. Values obtained from cells incubated in 5 mM glucose are considered as 100%.
