Table 1.
Cell Counts and Trypan Blue Exclusion Assay of Y79 Cells Exposed to Ultrasound Alone
| Before Ultrasound |
After Ultrasound |
|||||
|---|---|---|---|---|---|---|
| No. Cells Alive | No. Cells Dead | Total Cells | No. Cells Alive | No. Cells Dead | Total Cells | |
| Control | 3.4 × 105 | 0 | 3.4 × 105 | 3.2 × 105 | 0 | 3.2 × 105 |
| 1 W/cm2 | 3.8 × 105 | 3000 | 3.83 × 105 | 3.17 × 105 | 3.3 × 104 | 3.5 × 105 |
| 5 W/cm2 | 3.43 × 105 | 1000 | 3.44 × 105 | 2.7 × 105 | 5.0 × 104 | 3.2 × 105 |
| 10 W/cm2 | 3.1 × 105 | 0 | 3.1 × 105 | 4.0 × 104 | 5.0 × 104 | 9.0 × 104 |
Ultrasound intensity >1 W/cm2 was directly toxic to Y79 cells; a 60% decrease in cell viability was seen from exposure to ultrasound alone at an intensity of 10 W/cm2.