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Ratio of signal increments after 1 M MnCl2 topical administration. The signal reached a peak in day 1 and decayed to control levels in 7 days. Topical administration of 1 M MnCl2 was conducted again on day 7. Significant enhancements were seen the following day (Day 7+1). Signals decayed in 2 days and returned to baseline in 7 days after the second Mn2+ administration (Days 7+2 and Days 7+7, respectively). * P < 0.05 comparing to control sites.
