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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Biochem Pharmacol. 2011 Apr 9;82(2):122–130. doi: 10.1016/j.bcp.2011.03.031

Figure 2. ICI dependent ERα degradation is saturable in breast cancer cell lines.

Figure 2

A) ER positive (MCF7, BT483, and BT474) and negative (MDA-MB-231) cells were infected with increasing MOI of ERα adenovirus prior to 24 hours treatment with E2 (100 nM) or ICI (1µM). ER levels were analyzed by immunoblotting of whole cell extracts (WCE). Corresponding immunoblots of loading control KRT18 are featured in Suppl. Figure1. Expression of ER target genes TFF1 or WISP2 in cells infected in parallel and treated 24 hours with E2 (10 nM) in the presence or absence of ICI (1 µM) was analyzed by qRT-PCR as in Figure 1 (illustrated below the corresponding immunoblots). B) MCF7 cells were infected with MOI of 100 ER adenovirus and treated with Veh, ICI, or Laso (1 µM) in the presence or absence of E2 (10 nM) for 24 hours prior to immunoblot analysis of ERα. Data are representative of at least 3 independent experiments.