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. Author manuscript; available in PMC: 2012 Jun 30.
Published in final edited form as: Chem Biol Interact. 2011 Jan 13;192(1-2):118–121. doi: 10.1016/j.cbi.2011.01.006

Figure 3.

Figure 3

Reaction of 3,4-dihydroxyphenylacetaldehyde (DOPAL) but not dopamine (DA) or 3,4-dihydroxyphenylacetic acid (DOPAC) with glyceraldehyde-3-phosphate dehydrogenase yielded significant protein cross-linking/aggregation. Lane 1, molecular weight marker. Lane 2, control protein. Lane 3, protein with DA. Lane 4, protein with DOPAL. Lane 5, protein with DOPAC. The SDS-PAGE gel was stained with Coomassie. Solid arrows point to the protein monomer (~38 kD) and the open arrow denotes protein aggregates (>200 kD). Note the disappearance of protein monomer in Lane 4 (DOPAL treatment) that correlates with protein aggregation.