Figure 7.

Northern blot analysis of the expression of the DLGR-2 gene at several developmental stages. Marker size (left) is given in kb. (A) Poly(A)⁺ RNA from each developmental stage was hybridized with a cDNA fragment coding for the seven-transmembrane domain of DLGR-2. This Northern blot shows that mRNA is only present in embryos and pupae, not in larvae and adult (mixed male and female) flies. (B) Hybridization of the same blot as in A with a cDNA probe coding for RP49. The RP49 gene is regarded to be expressed in all developmental stages (O'Connell and Rosbash 1984; Kerrebrock et al. 1995).