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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2011 Mar 17;51(1):197–204. doi: 10.1016/j.freeradbiomed.2011.03.018

Fig. 1.

Fig. 1

Impact of SOD1 knockout on hepatic GPX1 activity (A), protein amount (B) and co-immunopreciptation of hepatic GPX1 and SOD1 (C). A. Hepatic GPX1 activity was expressed as mean ± SE (n = 5). B. Western blot analysis of hepatic GPX1 protein (10 μg protein/lane). The image is a representative of three independent experiments. C. Co-immunoprecipation of GPX1 and SOD1 in liver homogenates. After 100 μg of total liver homogenate protein was incubated with 1 μg anti-GPX1 or anti-SOD1 antibody overnight at 4°C. The precipitates were loaded to SDS-PAGE (12% gel) and transferred to membranes that were blotted against the designated antibodies. WT liver homogenate was used as the positive control for Western blot. The image is a representative of three independent experiments.