Table II. Candidate SNPs with p-values for Hardy-Weinberg Equilibrium and Minor Allele Frequencies.

(SNP=single nucleotide polymorphism; HWE=Hardy-Weinberg Equilibrium; MAF=minor allele frequency) Predicted MAF is based on the HapMap-CEU population. Observed MAF is derived from genotypes of the healthy volunteers (HV) in the full study cohort of 714 subjects (N=282) and the HV subset (N=56) of the 238 subjects with transit measurements. HWE p-value >0.05 denotes that the study sample is in HWE for the corresponding SNP; the two p-values not satisfying HWE are marked with *.

Gene	SNP rs ID	Major Allele	Minor Allele	Predicted MAF	HV in Full cohort N=282		HV in Transit cohort N=56		Function	Amino Acid Position	Amino Acid Change
					Observed MAF	HWE p-value	Observed MAF	HWE p-value
SHP	rs6659176	C	G	0.092	0.061	0.266	0.055	1.000	missense	171	Ala ⇒ Gly
ASBT	rs188096	G	T	0.192	0.111	0.759	0.164	1.000	missense	171	Ala ⇒ Ser
KLB	rs17618244	G	A	0.155	0.181	0.109	0.164	0.325	missense	728	Arg ⇒ Gln
KLB	rs4975017	C	A	0.325	0.355	0.513	0.409	0.100	missense	1020	Gln ⇒ Lys
FGFR4	rs1966265	G	A	0.224	0.226	0.309	0.200	0.670	missense	10	Val ⇒ Ile
FGFR4	rs376618	T	C	0.225	0.204	0.001 *	0.273	0.047 *	missense	136	Leu ⇒ Pro
FGFR4	rs351855	G	A	0.283	0.333	0.079	0.318	0.210	missense	388	Gly ⇒ Arg
OSTα	rs939885	G	A	0.458	0.470	1.000	0.518	0.279	missense	202	Val ⇒ Ile
OSTβ	rs2946676	A	G	0.14	0.142	0.087	0.100	1.000	near-gene-3 (dbSNP)
OSTβ	rs4238399	C	T	0.37	0.407	0.324	0.445	1.000	near-gene-3 (dbSNP)
CYP7A1	rs8192879	C	T	0.37	0.407	0.535	0.336	0.362	utr-3(dbSNP)
CYP7A1	rs8192877	A	G	0.18	0.147	0.473	0.173	0.648	intron(dbSNP)
CYP7A1	rs11786580	C	T	0.24	0.197	0.347	0.227	0.711	intron(dbSNP)
CYP7A1	rs7833904	T	A	0.41	0.394	0.079	0.373	0.563	intergenic(GVS)
CYP7A1	rs10957057	C	T	0.14	0.127	0.277	0.100	1.000	intergenic(GVS)