Table 2.
Sequences and Characteristics of ET Primers
| Marker name | Chromosome location | Repeat | PCR product size range (bps) | Forward primer sequence | Reverse primer sequence |
|---|---|---|---|---|---|
| IFN-A | 9p23-9p22 | di (gt) | 130–155 | TGCGCGTTAAGTTAATTGGTT | M′GTAAGGT*GGAAACCCCCACT |
| D9S171 | 9p21-pp21 | di (ca) | 150–185 | AGCTAAGTGAACCTCATCTCTGTCT | M′ACCCT*AGCACTGATGGTATAGTCT |
| D9S162 | 9p22-9p21 | di (ca) | 160–205 | M′GCAAT*GACCAGTTAAGGTTC | AATTCCCACAACAAATCTCC |
| D9S747 | 9pter-9pter | tetra (gata) | 170–210 | GCCATTATTGACTCTGGAAAAGAC | M′CAGGCT*CTCAAAATATGAACAAAAT |
(M′) Indicates the location of conjugation to the common cyanine donor dye (CYA).
(T*) Indicates the modified T nucleotide where the acceptor dyes, ROX or R110, were conjugated. (Hung et al. 1996; Hung et al. 1998; Ju et al. 1995)