Table 2.
Primers used for PCR amplification of genes in the Open Biosystems Yeast deletion library: | |
Hsc82F | 5′-AAGCGTTGGGTAATGAGGGA |
Hsc82R | 5′-GGACAGCTGGTAGGACAATTT |
Hsp82F | 5′-TAATACCAACCAGGTCCTTCC |
Hsp82R | 5′-CGATTTCAGATTCTTCGCGT |
Cox4F | 5′-CAGATCGAACGAAACGGAAA |
Cox4R | 5′-AACTTCGCAACAACCTCTAGC |
Primers used for PCR amplification and sequencing of RPT3: | |
Rpt3F | 5′-TGGACAAACCAATTCTGCCT |
Rpt3R | 5′-CGTGATAAAAAAGTGGCGTC |
Rpt3seq1 | 5′-GGCAATCGGATAAAATTC |
Rpt3seq2 | 5′-GAACCTATTGATCAGAAC |
Rpt3seq3 | 5′-CCGGTAAGACGATGCTTG |
Rpt3seq4 | 5′-GATTAGATAGAAAGATTGAG |
Primers used for RT-PCR amplification of ~150 base segments of HSC82, HSP82, and ACT1: | |
Hsc82F | CTGCCATCAGAACTGGTCAA |
Hsc82R | TATCTTGTGCACCACCCTCA |
Hsp82F | ACTCCAAAGCCAGAGCAAAA |
Hsp82R | ACCTGAACTCTGTCGGCAAC |
Act1F | GCCTTCTACGTTTCCATCCA |
Act1R | GGCCAAATCGATTCTCAAAA |