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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2011 Mar 30;51(1):88–96. doi: 10.1016/j.freeradbiomed.2011.03.027

Figure 3. Western blot analysis of NSC-34/G93A A) Cell lysates for Nrf2/ARE proteins. B) Cytoplasmic and nuclear fractions for Nrf2 protein.

Figure 3

Figure 3

NSC-34/G93A cells were plated and treated with CDDO-TFEA or DMSO as described in Figure 2. Cell lysates were prepared at the indicated time points. A) Lanes 1, 3, and 5 each contain 30 µg of total lysates from CDDO-TFEA-treated cells and lanes 2, 4, and 6 each contain 30 µg of total lysates from DMSO-treated cells. Nrf2 is upregulated as early as 8 hours. NQO1, HO1 and GR were upregulated and reached peak levels at 48 hours. Beta-actin, a house-keeping protein is shown for a loading control. B) Lanes 1 and 3 are cytoplasmic (C) and lanes 2 and 4 are nuclear (N) fractions, 20 µg per lane loaded. . SOD1 used as cytoplasmic protein.