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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2011 Mar 30;51(1):88–96. doi: 10.1016/j.freeradbiomed.2011.03.027

Figure 4. Nrf2 upregulation and translocation in rat embryonic motor neurons in response to CDDO-TFEA.

Figure 4

Rat embryonic motor neurons (Rat eMNs) were isolated as described in the Methods section. Rat eMNs were plated and treated with 300 nM CDDO-TFEA (Figure 4B, D) or DMSO (Figure 4A, C) for 24 hours. These cells were washed, fixed and then stained for Nrf2 using anti-Nrf2 (Figure 4A,B). Double immunofluorescence was performed to study nuclear translocation of Nrf2. CDDO-TFEA incubation led to a reduction of cytoplasmatic Nrf2 staining (green fluorescence) and increased nuclear colocalization (Figure 4 C,D).