Figure 2.

Bipyridyl treatment modulates eukaryotic iron-responsive transcription. Human epithelial HEp2 cells were incubated in complete medium containing 100 μM DFO or Bpdl for 10 h before select transcript levels were measured via RT-qPCR. Reported iron-responsive genes, transferrin receptor (TfR) and divalent metal transporter 1 (DMT1), and iron-repressed genes, ferritin heavy and light chain (Fer-H/L) were normalized to endogenous glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA levels and then calibrated to the untreated control (ΔΔCt method; Pfaffl, 2001). The y-axis presents the natural log of the target gene relative expression. Bars represent the natural log of mean relative expressions ± SD in one representative experiment containing two biological replicates per treatment, each assayed in triplicate. Experiment was performed independently two times and obtained similar transcriptional profiles in each case (i.e., total of four biological replicates were observed). Statistical significance from the untreated was determined by unpaired, one-tailed t-test (*p < 0.05, **p < 0.01).