Figure 5.

Viability of C. trachomatis cultured with Bipyridyl. (A) Chlamydia-specific DNA was quantified via RT-qPCR from total genomic DNA extracted each 6 h. The numbers of chlamydial genomes (y-axis) were extrapolated from a standard curve of genomic DNA obtained from purified chlamydial EBs. Data points represent the mean ± SD of two independent experiments, which were representative of greater than five independent time course experiments. (B) Bpdl-cultured C. trachomatis were reactivated by washing and incubation with normal, iron-replete medium (“18 + 18” and “24 + 24”) and compared to cultures treated with Bpdl throughout the entire experiment (“24,” “36,” “48”). C. trachomatis treated with Bpdl for 18 and 24 h were still viable when cultured with normal media. Graph represents data combined from two independent experiments, each assayed in triplicate. Bars represent mean ± SD.