Figure 5.

Effect of cytochalasin D on E. coli O157:H7 association with and internalization by the PBRE cells. The PBRE cell monolayers were pretreated with cytochalasin D for 30 min before the addition of E. coli O157:H7 at 100 MOI and incubated for 4 h in the presence of the inhibitor. (A) Total cell-associated bacteria were quantified after PBRE cell-lysis with 0.1% (v/v) Triton X-100. (B) Intracellular bacteria were quantified after gentamicin treatment for 2 h. Results are expressed as cell-associated or intracellular bacteria relative to those obtained without inhibitor, taken as 100%. Each value is the mean of three separate experiments.