Table 1.
A combined list of genes essential for F. tularensis lethality to adult fruit flies.
| Gene loci (U112) | Gene product | Gene | Ahlund et al. (2010) | Asare et al. (2010) | Moule et al. (2010) |
|---|---|---|---|---|---|
| FTN_0019 | Aspartate carbamoyltransferase | pyrB | × | ||
| FTN_0020 | Carbamoyl-phosphate synthase large chain | carB | × | ||
| FTN_0021 | Carbamoyl-phosphate synthase small chain | carA | × | ||
| FTN_0024 | Dihydroorotase | pyrC | × | ||
| FTN_0030 | Hypothetical membrane protein | × | |||
| FTN_0035 | Orotidine-5-phosphate decarboxylase | pyrF | × | × | |
| FTN_0036 | Dihydroorotate dehydrogenase | pyrD | × | × | |
| FTN_0038 | Hypothetical protein | × | |||
| FTN_0051 | Conserved protein of unknown function | × | |||
| FTN_0052 | Protein of unknown function | × | |||
| FTN_0063 | Branched-chain amino acid aminotransferase protein (class IV) | ilvE | * | × | |
| FTN_0066 | Ferrous iron transport protein B | feoB | × | ||
| FTN_0068 | Oligoribonuclease | orn | × | ||
| FTN_0077 | Protein of unknown function | × | |||
| FTN_0078 | Shikimate 5-dehydrogenase | aroE1 | × | ||
| FTN_0090 | Acid phosphatase (precursor) | acpA | × | ||
| FTN_0096 | Conserved hypothetical membrane protein | × | |||
| FTN_0097 | Hydroxy/aromatic amino acid permease (HAAAP) family protein | × | |||
| FTN_0101 | Transcription regulator | × | |||
| FTN_0107 | GTP-binding protein LepA | lepA | × | ||
| FTN_0109 | Protein of unknown function | × | × | ||
| FTN_0111 | Riboflavin synthase beta-chain | ribH | × | ||
| FTN_0113 | Riboflavin synthase alpha chain | ribC | × | ||
| FTN_0115 | Overlaps Na+/H+ antiporter NHAP, fragment | × | × | ||
| FTN_0124 | Single-strand DNA binding protein | ssb | × | ||
| FTN_0141 | ABC transporter, ATP-binding protein | × | |||
| FTN_0162 | Cell division protein FtsQ | ftsQ | × | ||
| FTN_0190 | Major facilitator superfamily (MFS) transport protein, fragment | × | |||
| FTN_0192 | Cytochrome d terminal oxidase, polypeptide subunit II | cydB | × | ||
| FTN_0207 | Protein of unknown function containing a von Willebrand factor type A (vWA) domain | × | |||
| FTN_0214 | Valyl-tRNA synthetase | valS | × | ||
| FTN_0217 | l-lactate dehydrogenase | lldD | × | ||
| FTN_0266 | Chaperone Hsp90, heat shock protein HtpG | htpG | × | ||
| FTN_0275 | Hypothetical protein | × | |||
| FTN_0330 | Septum formation inhibitor-activating ATPase | minD | × | × | |
| FTN_0331 | Septum formation inhibitor | minC | × | ||
| FTN_0337 | Fumarate hydratase, class I | fumA | × | ||
| FTN_0338 | MutT/nudix family protein | × | |||
| FTN_0344 | Aspartate:alanine antiporter | × | |||
| FTN_0346 | OmpA family protein | × | |||
| FTN_0384 | Conserved hypothetical protein | × | |||
| FTN_0392 | Transcriptional regulator, LysR family | × | |||
| FTN_0404 | Peptide methionine sulfoxide reductase | msrB | × | ||
| FTN_0409 | Alcohol dehydrogenase class III, pseudogene | × | |||
| FTN_0412 | DNA repair protein recN | × | |||
| FTN_0416 | Lipid A 1-phosphatase | lpxE | × | ||
| FTN_0429 | Hypothetical protein | × | |||
| FTN_0439 | Protein of unknown function | × | |||
| FTN_0482 | Protein of unknown function | × | |||
| FTN_0493 | 5-Methylthioadenosine/S-adenosylhomocysteine nucleosidase | mtn | × | ||
| FTN_0494 | Hypothetical membrane protein | × | |||
| FTN_0495 | BNR/Asp-box repeat protein | × | |||
| FTN_0496 | Soluble lytic murein transglycosylase | slt | × | ||
| FTN_0504 | Lysine decarboxylase | × | |||
| FTN_0507 | Glycine cleavage system P protein, subunit 1 | gcvP1 | × | ||
| FTN_0513 | 1,4-α-Glucan branching enzyme | glgB | × | ||
| FTN_0516 | Glycogen synthase | glgA | × | ||
| FTN_0525 | Bifunctional aspartokinase/homoserine dehydrogenase I, pseudogene | thrA | × | ||
| FTN_0526 | Homoserine kinase (pseudogene) | thrB | × | ||
| FTN_0538 | Conserved hypothetical membrane protein | × | |||
| FTN_0545 | Glycosyl transferase, group 2 | × | |||
| FTN_0546 | Dolichyl-phosphate-mannose-protein mannosyltransferase family protein | × | |||
| FTN_0549 | Stringent starvation protein A | sspA | × | ||
| FTN_0554 | tRNA/rRNA methyltransferase | yibK | × | ||
| FTN_0567 | tRNA synthetase class II (D, K and N) | × | |||
| FTN_0577 | DNA mismatch repair enzyme with ATPase activity | mutL | × | ||
| FTN_0588 | Asparaginase | × | |||
| FTN_0593 | Succinyl-CoA synthetase, alpha subunit | sucD | × | ||
| FTN_0599 | Conserved hypothetical protein | × | |||
| FTN_0599 | Protein of unknown function | × | |||
| FTN_0600 | DNA gyrase subunit B | gyrB | × | ||
| FTN_0603 | Formamidopyrimidine-DNA glycosylase | mutM | × | ||
| FTN_0623 | 2-C-methyl-d-erythritol 4-phosphate cytidylyltransferase | ispD | × | ||
| FTN_0627 | Chitinase, glycosyl hydrolase family 18 | chiA | × | ||
| FTN_0649 | FAD-binding family protein, pseudogene | × | |||
| FTN_0651 | Cytidine deaminase | cdd | × | ||
| FTN_0652 | Uridine phosphorylase | udp | × | ||
| FTN_0653 | tRNA-(ms(2)io(6)a)-hydroxylase | miaE | × | ||
| FTN_0655 | Methylase | × | |||
| FTN_0660 | Cytosol aminopeptidase | pepA | × | ||
| FTN_0664 | Type IV pili fiber building block protein | × | |||
| FTN_0666 | Excinuclease ABC, subunit A | uvrA | × | ||
| FTN_0667 | Major facilitator superfamily (MFS) transport protein | yieO | × | ||
| FTN_0672 | Preprotein translocase, subunit A | secA | × | ||
| FTN_0673 | DNA-3-methyladenine glycosylase I (pseudogene) | tag | × | ||
| FTN_0692 | Quinolinate sythetase A | nadA | × | ||
| FTN_0696 | Hypothetical membrane protein | × | |||
| FTN_0728 | Predicted Co/Zn/Cd cation transporter | × | |||
| FTN_0732 | Hypothetical protein | × | |||
| FTN_0741 | Proton-dependent oligopeptide transporter (POT) family protein, di- or tripeptide:H+ symporter | × | |||
| FTN_0750 | l-Serine dehydratase 1 | sdaA | × | ||
| FTN_0759 | Conserved hypothetical protein | × | |||
| FTN_0760 | Conserved hypothetical protein | × | |||
| FTN_0768 | Tryptophan-rich sensory protein | tspO | × | ||
| FTN_0770 | Major facilitator superfamily (MFS) transport protein, pseudogene | bcr1 | × | ||
| FTN_0772 | Conserved protein of unknown function | × | |||
| FTN_0773 | 4Fe–4S ferredoxin (electron transport) family protein, pseudogene | yjeS | × | ||
| FTN_0774 | Conserved hypothetical protein | × | |||
| FTN_0781 | Transaldolase | talA | × | ||
| FTN_0783 | Isochorismatase hydrolase family protein | × | |||
| FTN_0790 | Recombination associated protein | rdgC | × | ||
| FTN_0791 | Protein of unknown function | × | |||
| FTN_0806 | Glycosyl hydrolase family 3 | × | |||
| FTN_0810 | ROK family protein | × | |||
| FTN_0824 | Major facilitator superfamily (MFS) transport protein, pseudogene | × | |||
| FTN_0826 | Aldo/keto reductase | × | |||
| FTN_0838 | Exodeoxyribonuclease III | xthA | × | ||
| FTN_0840 | Modulator of drug activity B | mdaB | × | × | |
| FTN_0848 | Amino acid antiporter | × | × | ||
| FTN_0855 | Protein of unknown function | × | |||
| FTN_0861 | Type IV pili fiber building block protein | pilA | × | × | |
| FTN_0875 | Major facilitator superfamily (MFS) transport protein | × | |||
| FTN_0877 | Cardiolipin synthetase | cls | × | ||
| FTN_0885 | Proton-dependent oligopeptide transporter (POT) family protein | yhiP | × | ||
| FTN_0886 | Hypothetical membrane protein | × | |||
| FTN_0887 | Hypothetical protein | × | |||
| FTN_0888 | Hypothetical membrane protein | × | |||
| FTN_0889 | Helix-turn-helix family protein | × | |||
| FTN_0891 | Holliday junction DNA helicase, subunit B | ruvB | × | ||
| FTN_0898 | Amino acid permease | × | |||
| FTN_0900 | Protein of unknown function with predicted hydrolase and phosphorylase activity | × | |||
| FTN_0921 | FKBP-type peptidyl-prolyl cis-trans isomerase | × | |||
| FTN_0928 | Sulfate adenylyltransferase subunit 2 | cysD | × | ||
| FTN_0949 | 50S ribosomal protein L9 | rplI | × | ||
| FTN_0954 | Histidine acid phosphatase | × | |||
| FTN_0959 | Oxidative stress transcriptional regulator | oxyR | × | ||
| FTN_0972 | Hypothetical protein | × | |||
| FTN_0975 | Hypothetical protein | × | |||
| FTN_0976 | ThiF family protein, pseudogene | × | |||
| FTN_0978 | Ubiquinone biosynthesis protein | × | |||
| FTN_0982 | Glutaredoxin 1 | grxA | × | ||
| FTN_0984 | ABC transporter, ATP-binding protein | × | |||
| FTN_0997 | Proton-dependent oligopeptide transporter (POT) family protein, di- or tripeptide:H+ symporter | × | |||
| FTN_1006 | Transporter-associated protein, HlyC/CorC family | × | |||
| FTN_1014 | Nicotinamide mononucleotide transport (NMT) family protein | × | |||
| FTN_1016 | Hypothetical protein | × | |||
| FTN_1026 | Major facilitator superfamily (MFS) transport protein, pseudogene | × | |||
| FTN_1027 | Holliday junction endodeoxyribonuclease | ruvC | × | × | |
| FTN_1034 | Iron-sulfur cluster-binding protein | rnfB | × | ||
| FTN_1038 | Conserved hypothetical membrane protein | × | |||
| FTN_1058 | Trigger factor (TF) protein | tig | × | ||
| FTN_1066 | Metal ion transporter protein | × | |||
| FTN_1073 | DNA/RNA endonuclease G | × | |||
| FTN_1091 | 3-Phosphoshikimate 1-carboxyvinyltransferase | aroA | × | ||
| FTN_1099 | Transcriptional regulator, LysR family | × | |||
| FTN_1115 | Type IV pili nucleotide binding protein, ABC transporter, ATP-binding protein | pilB | × | ||
| FTN_1116 | Type IV pili polytopic inner membrane protein | pilC | × | ||
| FTN_1135 | 3-Dehydroquinate synthetase | aroB | × | ||
| FTN_1137 | Type IV pilin multimeric outer membrane protein | pilQ | × | × | |
| FTN_1168 | Exodeoxyribonuclease VII large subunit | xseA | × | ||
| FTN_1170 | Conserved protein of unknown function | × | |||
| FTN_1171 | Conserved hypothetical lipoprotein | × | |||
| FTN_1174 | Glutamate racemase | murI | × | ||
| FTN_1176 | Excinuclease ABC, subunit B | uvrB | × | ||
| FTN_1179 | Transcriptional regulator, LysR family | × | |||
| FTN_1186 | Metallopeptidase family M13 protein, pseudogene | × | × | ||
| FTN_1196 | Conserved hypothetical UPF0133 protein | ybaB | × | ||
| FTN_1198 | Guanosine-3,5-bis(diphosphate) 3-pyrophosphohydrolase/(p)ppGpp synthase | spoT | × | ||
| FTN_1214 | Glycosyl transferase, family 2 | × | |||
| FTN_1215 | Capsule polysaccharide export protein KpsC | kpsC | × | ||
| FTN_1220 | Bacterial sugar transferase family protein | × | |||
| FTN_1223 | Conserved hypothetical membrane protein | × | |||
| FTN_1240 | BolA family protein | × | |||
| FTN_1241 | DedA family protein | × | |||
| FTN_1243 | DNA repair protein recO | recO | × | ||
| FTN_1257 | Membrane protein of unknown function | × | |||
| FTN_1261 | Protein of unknown function | × | |||
| FTN_1268 | Mycobacterial cell entry (mce) related family protein | × | |||
| FTN_1275 | Drug:H+ antiporter-1 (DHA2) family protein | × | |||
| FTN_1276 | Membrane fusion protein | × | |||
| FTN_1278 | NH(3)-dependent NAD(+) synthetase | nadE | × | ||
| FTN_1282 | LysR transcriptional regulator family protein | × | |||
| FTN_1290 | Macrophage growth locus, subunit A | mglA | × | ||
| FTN_1291 | Macrophage growth locus, subunit B | mglB | × | ||
| FTN_1300 | LysR transcriptional regulator family protein | × | |||
| FTN_1309 | Protein of unknown function | pdpA | × | ||
| FTN_1310 | Conserved hypothetical protein; conserved hypothetical protein | pdpB; pdpB1 | × | × | |
| FTN_1311 | Protein of unknown function | iglEa | × | ||
| FTN_1312 | Conserved hypothetical protein | vgrGa | × | ||
| FTN_1313 | Hypothetical protein | iglFa | × | ||
| FTN_1314 | Conserved hypothetical protein | iglGa | × | ||
| FTN_1315 | Protein of unknown function | iglHa | × | ||
| FTN_1316 | Conserved protein of unknown function | dotUa | × | ||
| FTN_1317 | Protein of unknown function | iglIa | × | ||
| FTN_1318 | Hypothetical protein | iglJa | × | ||
| FTN_1319 | Conserved hypothetical protein; conserved hypothetical protein | pdpC | × | × | |
| FTN_1321 | Intracellular growth locus, subunit D; subunit D | iglD; iglD1 | × | × | × |
| FTN_1322 | Intracellular growth locus, subunit C; subunit C1 | iglC; iglC1 | × | * | × |
| FTN_1323 | Conserved protein of unknown function | iglB | × | ||
| FTN_1324 | Conserved protein of unknown function | iglA | × | ||
| FTN_1343 | Conserved protein of unknown function | × | |||
| FTN_1357 | ATP-dependent exoDNAse (exonuclease V) beta subunit | recB | × | × | |
| FTN_1359 | Exodeoxyribonuclease V gamma chain | recC | × | ||
| FTN_1368 | Fe2+ transport system protein A | feoA | × | ||
| FTN_1372 | Protein of unknown function | × | |||
| FTN_1376 | Disulfide bond formation protein, DsbB family | × | |||
| FTN_1386 | Protein of unknown function | × | |||
| FTN_1406 | Conserved hypothetical membrane protein | x | |||
| FTN_1409 | Major facilitator superfamily (MFS) transport protein, pseudogene | × | × | ||
| FTN_1412 | DNA-directed RNA polymerase e subunit | x | |||
| FTN_1417 | Phosphomannomutase | manB | × | × | |
| FTN_1428 | Transferase | wbtO | × | ||
| FTN_1439 | 3-Ketoacyl-CoA thiolase | fadA | × | ||
| FTN_1441 | Sugar transport protein, pseudogene | × | × | ||
| FTN_1448 | Protein of unknown function | × | |||
| FTN_1452 | Two-component response regulator | × | |||
| FTN_1457 | Protein of unknown function | × | |||
| FTN_1465 | Two-component response regulator | pmrA | × | ||
| FTN_1488 | Prophage maintenance system killer protein (DOC) | × | |||
| FTN_1491 | Adenine specific DNA methylase | × | |||
| FTN_1494 | Pyruvate dehydrogenase complex, E1 component, pyruvate dehydrogenase | aceE | × | ||
| FTN_1501 | Monovalent cation:proton antiporter-1 | × | |||
| FTN_1513 | Site-specific recombinase | xerC | × | ||
| FTN_1518 | GDP pyrophosphokinase/GTP pyrophosphokinase | relA | × | ||
| FTN_1530 | Diaminopimelate decarboxylase | lysA | × | ||
| FTN_1534 | Conserved protein of unknown function | × | |||
| FTN_1542 | Conserved protein of unknown function | × | |||
| FTN_1549 | Drug:H+ antiporter-1 (DHA1) family protein | × | |||
| FTN_1552 | Acid phosphatase, PAP2 family | × | |||
| FTN_1557 | Oxidoreductase iron/ascorbate family protein | × | |||
| FTN_1580 | Helicase | × | |||
| FTN_1582 | Hypothetical membrane protein | × | |||
| FTN_1584 | Glycerol-3-phosphate dehydrogenase | glpD | × | ||
| FTN_1593 | ABC-type oligopeptide transport system, periplasmic component | oppA | × | ||
| FTN_1595 | Signal recognition particle receptor FtsY | ftsY | × | ||
| FTN_1599 | Nucleoside permease NUP family protein | nupC | × | ||
| FTN_1600 | Nucleoside permease NUP family protein | nupC1 | × | ||
| FTN_1608 | Disulfide bond formation protein | dsbB | × | ||
| FTN_1611 | Major facilitator superfamily (MFS) transport protein | – | × | ||
| FTN_1612 | Hypothetical protein | × | |||
| FTN_1617 | Sensor histidine kinase | qsec | × | ||
| FTN_1618 | Conserved hypothetical protein | × | |||
| FTN_1621 | Predicted NAD/FAD-dependent oxidoreductase | × | |||
| FTN_1630 | Preprotein translocase, subunit G, membrane protein | secG | × | ||
| FTN_1654 | Major facilitator superfamily (MFS) transport protein | × | |||
| FTN_1657 | Major facilitator superfamily (MFS) transport protein | × | |||
| FTN_1658 | Histidyl-tRNA synthetase | hisS | × | ||
| FTN_1678 | NADH dehydrogenase I, C subunit | nuoC | × | ||
| FTN_1682 | Conserved hypothetical protein | × | |||
| FTN_1683 | Drug:H+ antiporter-1 (DHA1) family protein | × | |||
| FTN_1685 | Drug:H+ antiporter-1 (DHA1) family protein | × | |||
| FTN_1692 | Secretion protein | × | |||
| FTN_1704 | Protein-l-isoaspartate O-methyltransferase | pcm | × | ||
| FTN_1714 | Transcriptional regulatory protein, pseudogene | kdpE | × | ||
| FTN_1715 | Two-component sensor protein | kdpD | × | ||
| FTN_1716 | Potassium-transporting ATPase C chain | kdpC | × | ||
| FTN_1718 | Potassium-transporting ATPase, A chain, pseudogene | kdpA | × | ||
| FTN_1719 | NAD-dependent formate dehydrogenase, fragment | × | |||
| FTN_1733 | Conserved hypothetical membrane, pseudogene | × | |||
| FTN_1743 | Chaperone ClpB | clpB | × | ||
| FTN_1745 | Phosphoribosylglycinamide formyltransferase 2 | purT | × | ||
| FTN_1750 | Acyltransferase | × | |||
| FTN_1753 | Oxidase-like protein, pseudogene | × | |||
| FTN_1762 | ABC transporter, ATP-binding protein | yjjK | × | ||
| FTN_1763 | Major facilitator superfamily (MFS) transport protein | × | |||
| FTN_1776 | Anthranilate synthase component II, pseudogene | trpG1 | × |
An × marks a screen, where the gene was identified to be essential for lethality to flies, whereas * marks a gene not essential for lethality to flies, but the gene is important for reduction of bacterial load in the indicated screen (mentioned because it was found in at least one other screen). Genes found in multiple screens are shown in a bold font.