Table 1.
Primer name | Primer sequence | Targeted gene | Product size [bp] | Melting temp. [°C] |
---|---|---|---|---|
ACA0302For | TTCGAGCAACTCCTGCAGACG | sor | 271 | 55.5 |
ACA0302Rev | CGTCCGTCATACCCATGATCC | ACA_0302 | ||
ACA1632For | GATCCAGGCGATTCATATACGG | doxD | 337 | 55.5 |
ACA1632Rev | TGATCCCCATAGCGAAATTAGAG | ACA_1632 | ||
ACA1633For | TTTTGCGCGTTTGTACCTACCC | tth | 223 | 55.5 |
ACA1633Rev | AACGCCGTCTACTTGAGCTCC | ACA_1633 | ||
ACA2312For | TGGCGATCTTACCTTGAGCGAGG | soxA-I | 436 | 55.5 |
ACA2312Rev | TGCGCTCTGCCCCAAAAGTGG | ACA_2312 | ||
ACA2392For | ATCTACCCTTCGACAAGTATGC | soxA-II | 527 | 55.5 |
ACA2392Rev | TGTGCCGTCTCGCCTTGCAAG | ACA_2392 | ||
ACA2317For | GAAGCCGGTACTGATCAACAAG | soxB-I | 437 | 55.5 |
ACA2317Rev | CGTGTACTCCGTAACCGTAACC | ACA_2317 | ||
ACA2394For | TCCGTCCTCAACCAGACGCCC | soxB-II | 467 | 55.5 |
ACA2394Rev | ACAGCGATTTTGCGACCGCTG | ACA_2394 | ||
ACA2319For | CTCGCGCCGCAAATGTTCCCG | soxY-I | 180 | 55.5 |
ACA2319Rev | TGATCGACATCCACGGTAACCG | ACA_2319 | ||
ACA2390For | ATCGGCACAAGCCTCGTTGCG | soxY-II | 340 | 55.5 |
ACA2390Re2 | CTGGGGTGAGATCGAACTGGC | ACA_2390 | ||
ACA2313For | GTGCAGTTTATTTACGACCCGG | soxX-I | 98 | 58 |
ACA2313Rev | ACCAAGCCAATCTGGTGATCCG | ACA_2313 | ||
ACA2389For | ACTCGATACCATCGTTCGTGC | soxX-II | 256 | 58 |
ACA2389Rev | TCTGGAACATCTGCTGGAAGG | ACA_2389 | ||
ACA2318For | AGAGGTCCGTTCGTTGATCATG | soxZ-I | 269 | 58 |
ACA2318Rev | TCAGGCGACGGTGATCTTTGCC | ACA_2318 | ||
ACA2391For | ATGGCAGACAATATTGGTAACCC | soxZ-II | 197 | 58 |
ACA2391Rev | TCGATGTCCAGCAGCTTTGCAC | ACA_2391 | ||
ACAgyrA-F4 | CAGCCTCGAAAAAGAAATGC | gyrA | 431 | 55.5 |
ACAgyrA-R4 | CCACCTCCTTCTCGTCGTAG | ACA_1592 |