Table TA2.

Proteins identified in 2D gels from sessile and planktonic Acidithiobacillus caldus.

			Theoretical		Experimental		Mowse scoreg	Coverageh (%)	Fold
Match IDa	Accessionb	Protein identification	MW (kDa)c	pId	MW (kDa)e	pIf			E-valuei	differencej	Anovak
ACIDITHIOBACILLUS CALDUS PLANKTONIC
64	ACA_1726	Twin-arginine translocation protein TatA	8	6.40	16	6.33	71	51	2.1e − 04	7.9	9.0e − 03
118	ACA_2219	Hypothetical protein ACA_2219	20	6.15	20	6.11	75	31	8.9e − 05	4.2	1.4e − -03
492	ACA_0303	Sulfide–quinone reductase, sqr-1	47	5.57	52	5.56	86	35	6.5e − 06	2.4	7.2e − 5
826	ACA_0867	Hypothetical protein ACA_0867	18	9.07	14	7.18	101	61	2.2e − 07	unique	2.9e − 6
827	ACA_0867	Hypothetical protein ACA_0867	18	9.07	14	6.95	69	53	3.2e − 04	unique	5.2e − 5
841l	ACA_2319	Sulfur oxidation protein SoxY			16	5.65				unique	3.3e − 4
ACIDITHIOBACILLUS CALDUS SESSILE
70	ACA_1907	Single-stranded DNA-binding protein	17	5.49	16	5.59	70	66	3.0e − 04	3.1	3.1e − 02
213	ACA_1235	Peptidyl-prolyl cis-trans isomerase ppiD	28	8.54	27	7.12	56	20	6.6e − 03	2.7	1.1e − 02
238	ACA_2420	CoB–CoM heterodisulfide reductase subunit C	27	6.20	28	6.67	113	50	1.4e − 08	2.8	7.9e − 03
258	ACA_2765	Ribulose bisphosphate carboxylase large chain	53	5.96	30	5.36	68	15	4.7e − 04	2.2	5.1e − 4
280	ACA_2783	Rubisco activation protein CbbQ mod	30	5.32	32	5.32	84	35	1.2e − 05	2.4	5.3e − 5
325	ACA_2737	Twitching motility protein	39	6.50	36	6.87	102	33	1.8e − 07	2.0	1.7e − 03
329	ACA_2307	Heat shock protein 60 family chaperone GroEL	58	5.14	37	5.4	64	18	1.2e − 03	2.2	9.5e − 03
364	ACA_0002	Pyruvate dehydrogenase E1 component beta subunit	35	5.69	39	5.8	77	20	5.5e − 05	3.0	2.5e − 02
382	ACA_2418	Heterodisulfide reductase subunit A	38	6.03	40	5.57	93	34	1.5e − 06	2.4	3.6e − 4
	ACA_1473	Heterodisulfide reductase subunit A	38	5.86	40	5.57	68	28	4.3e − 04
395	ACA_2421	CoB–CoM heterodisulfide reductase subunit B	33	5.01	41	5.03	71	56	2.3e − 04	2.1	5.9e − 4
404	ACA_0152	40-residue YVTN family beta-propeller repeat protein	96	5.98	43	5.35	49	7	3.4e − 02	7.7	4.4e − 5
408	ACA_2100	Fructose-bisphosphate aldolase class II	38	5.69	43	5.93	76	33	7.4e − 05	2.3	1.6e − 02
420	ACA_0056	S-adenosylmethionine synthetase	42	5.37	45	5.38	79	21	3.3e − 05	2.4	9.0e − 6
442	ACA_0500	Gamma-glutamyl phosphate reductase	46	5.87	48	6.37	109	25	3.6e − 08	2.5	1.7e − 03
447	ACA_0113	Serine hydroxymethyltransferase	45	6.4	49	6.73	71	22	2.0e − 04	5.2	8.5e − 03
455	ACA_1035	3-isopropylmalate dehydratase large subunit	51	5.69	49	5.85	107	33	5.6e − 08	2.5	1.8e − 5
515	ACA_0148	2-isopropylmalate synthase	32	5.94	56	5.43	86	29	7.3e − 06	2.5	1.7e − 03
522	ACA_0976	ATP synthase alpha chain	56	5.32	57	5.27	68	18	4.0e − 04	2.3	1.7e − 03
541	ACA_2307	Heat shock protein 60 family chaperone GroEL	58	5.14	63	5.4	96	34	7.8e − 07	3.8	7.0e − 03
543	ACA_2307	Heat shock protein 60 family chaperone GroEL	58	5.14	67	5.26	94	28	1.2e − 06	2.4	8.1e − 4
553	ACA_2095	Transketolase	73	5.95	79	6.33	77	18	5.8e − 05	3.0	5.2e − 03
556	ACA_1454	Chaperone protein DnaK	68	5.06	82	5.02	155	36	8.9e − 13	2.6	3.4e − 03

^aMatch ID was generated by Melanie and refers to protein spots in Figure 5.

^bAnnotation number is that of Genebank genome annotation NZ_ACVD01000000.1.

^cPredicted Molecular weight of database entry.

^dPredicted isoelectric point (pI) of database entry.

^eExperimental molecular weight determined by 2D-PAGE.

^fExperimental pI determined by 2D-PAGE.

^gMowse score was generated by Mascot and describes the hit of a peptide mass fingerprint (PMF) search giving the probability that the hit is not a random match in the database (scores > 47 are significant with a significance threshold of 0.05).

^hSequence coverage (generated by Mascot) presents the percentage of the database hit that was covered by the submitted peptide masses of the experimentally acquired PMF.

ⁱExpect value (generated by Mascot) is the number of hits expected by change in a database of the same size.

^jFold change was determined with Melanie. Fold changes ≥2.0 were regarded as differentially expressed.

^kAnova test was performed for two or three replicates of each condition and spots with p-values < 0.05 were considered to be significant.

^lSample was analyzed by Edman degradation.