Table TA2.
Theoretical | Experimental | Mowse scoreg | Coverageh (%) | Fold | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Match IDa | Accessionb | Protein identification | MW (kDa)c | pId | MW (kDa)e | pIf | E-valuei | differencej | Anovak | ||
ACIDITHIOBACILLUS CALDUS PLANKTONIC | |||||||||||
64 | ACA_1726 | Twin-arginine translocation protein TatA | 8 | 6.40 | 16 | 6.33 | 71 | 51 | 2.1e − 04 | 7.9 | 9.0e − 03 |
118 | ACA_2219 | Hypothetical protein ACA_2219 | 20 | 6.15 | 20 | 6.11 | 75 | 31 | 8.9e − 05 | 4.2 | 1.4e − -03 |
492 | ACA_0303 | Sulfide–quinone reductase, sqr-1 | 47 | 5.57 | 52 | 5.56 | 86 | 35 | 6.5e − 06 | 2.4 | 7.2e − 5 |
826 | ACA_0867 | Hypothetical protein ACA_0867 | 18 | 9.07 | 14 | 7.18 | 101 | 61 | 2.2e − 07 | unique | 2.9e − 6 |
827 | ACA_0867 | Hypothetical protein ACA_0867 | 18 | 9.07 | 14 | 6.95 | 69 | 53 | 3.2e − 04 | unique | 5.2e − 5 |
841l | ACA_2319 | Sulfur oxidation protein SoxY | 16 | 5.65 | unique | 3.3e − 4 | |||||
ACIDITHIOBACILLUS CALDUS SESSILE | |||||||||||
70 | ACA_1907 | Single-stranded DNA-binding protein | 17 | 5.49 | 16 | 5.59 | 70 | 66 | 3.0e − 04 | 3.1 | 3.1e − 02 |
213 | ACA_1235 | Peptidyl-prolyl cis-trans isomerase ppiD | 28 | 8.54 | 27 | 7.12 | 56 | 20 | 6.6e − 03 | 2.7 | 1.1e − 02 |
238 | ACA_2420 | CoB–CoM heterodisulfide reductase subunit C | 27 | 6.20 | 28 | 6.67 | 113 | 50 | 1.4e − 08 | 2.8 | 7.9e − 03 |
258 | ACA_2765 | Ribulose bisphosphate carboxylase large chain | 53 | 5.96 | 30 | 5.36 | 68 | 15 | 4.7e − 04 | 2.2 | 5.1e − 4 |
280 | ACA_2783 | Rubisco activation protein CbbQ mod | 30 | 5.32 | 32 | 5.32 | 84 | 35 | 1.2e − 05 | 2.4 | 5.3e − 5 |
325 | ACA_2737 | Twitching motility protein | 39 | 6.50 | 36 | 6.87 | 102 | 33 | 1.8e − 07 | 2.0 | 1.7e − 03 |
329 | ACA_2307 | Heat shock protein 60 family chaperone GroEL | 58 | 5.14 | 37 | 5.4 | 64 | 18 | 1.2e − 03 | 2.2 | 9.5e − 03 |
364 | ACA_0002 | Pyruvate dehydrogenase E1 component beta subunit | 35 | 5.69 | 39 | 5.8 | 77 | 20 | 5.5e − 05 | 3.0 | 2.5e − 02 |
382 | ACA_2418 | Heterodisulfide reductase subunit A | 38 | 6.03 | 40 | 5.57 | 93 | 34 | 1.5e − 06 | 2.4 | 3.6e − 4 |
ACA_1473 | Heterodisulfide reductase subunit A | 38 | 5.86 | 40 | 5.57 | 68 | 28 | 4.3e − 04 | |||
395 | ACA_2421 | CoB–CoM heterodisulfide reductase subunit B | 33 | 5.01 | 41 | 5.03 | 71 | 56 | 2.3e − 04 | 2.1 | 5.9e − 4 |
404 | ACA_0152 | 40-residue YVTN family beta-propeller repeat protein | 96 | 5.98 | 43 | 5.35 | 49 | 7 | 3.4e − 02 | 7.7 | 4.4e − 5 |
408 | ACA_2100 | Fructose-bisphosphate aldolase class II | 38 | 5.69 | 43 | 5.93 | 76 | 33 | 7.4e − 05 | 2.3 | 1.6e − 02 |
420 | ACA_0056 | S-adenosylmethionine synthetase | 42 | 5.37 | 45 | 5.38 | 79 | 21 | 3.3e − 05 | 2.4 | 9.0e − 6 |
442 | ACA_0500 | Gamma-glutamyl phosphate reductase | 46 | 5.87 | 48 | 6.37 | 109 | 25 | 3.6e − 08 | 2.5 | 1.7e − 03 |
447 | ACA_0113 | Serine hydroxymethyltransferase | 45 | 6.4 | 49 | 6.73 | 71 | 22 | 2.0e − 04 | 5.2 | 8.5e − 03 |
455 | ACA_1035 | 3-isopropylmalate dehydratase large subunit | 51 | 5.69 | 49 | 5.85 | 107 | 33 | 5.6e − 08 | 2.5 | 1.8e − 5 |
515 | ACA_0148 | 2-isopropylmalate synthase | 32 | 5.94 | 56 | 5.43 | 86 | 29 | 7.3e − 06 | 2.5 | 1.7e − 03 |
522 | ACA_0976 | ATP synthase alpha chain | 56 | 5.32 | 57 | 5.27 | 68 | 18 | 4.0e − 04 | 2.3 | 1.7e − 03 |
541 | ACA_2307 | Heat shock protein 60 family chaperone GroEL | 58 | 5.14 | 63 | 5.4 | 96 | 34 | 7.8e − 07 | 3.8 | 7.0e − 03 |
543 | ACA_2307 | Heat shock protein 60 family chaperone GroEL | 58 | 5.14 | 67 | 5.26 | 94 | 28 | 1.2e − 06 | 2.4 | 8.1e − 4 |
553 | ACA_2095 | Transketolase | 73 | 5.95 | 79 | 6.33 | 77 | 18 | 5.8e − 05 | 3.0 | 5.2e − 03 |
556 | ACA_1454 | Chaperone protein DnaK | 68 | 5.06 | 82 | 5.02 | 155 | 36 | 8.9e − 13 | 2.6 | 3.4e − 03 |
aMatch ID was generated by Melanie and refers to protein spots in Figure 5.
bAnnotation number is that of Genebank genome annotation NZ_ACVD01000000.1.
cPredicted Molecular weight of database entry.
dPredicted isoelectric point (pI) of database entry.
eExperimental molecular weight determined by 2D-PAGE.
fExperimental pI determined by 2D-PAGE.
gMowse score was generated by Mascot and describes the hit of a peptide mass fingerprint (PMF) search giving the probability that the hit is not a random match in the database (scores > 47 are significant with a significance threshold of 0.05).
hSequence coverage (generated by Mascot) presents the percentage of the database hit that was covered by the submitted peptide masses of the experimentally acquired PMF.
iExpect value (generated by Mascot) is the number of hits expected by change in a database of the same size.
jFold change was determined with Melanie. Fold changes ≥2.0 were regarded as differentially expressed.
kAnova test was performed for two or three replicates of each condition and spots with p-values < 0.05 were considered to be significant.
lSample was analyzed by Edman degradation.