Table 5.
Up-regulated human proteins in infected vs. uninfected HL-60 cells by quantitative proteomics analysis1.
| Functional role category | Anaplasma phagocytophilum-infected vs. uninfected HL-60 cells | Ehrlichia chaffeensis-Infected vs. Uninfected HL-60 cells |
|---|---|---|
| Cytoskeleton components | Keratin; Arp2/3 protein complex; α-actinin-4; galectin-9; plastin-2 | Actin; vimentin; α-actinin-4; Arp2/3 protein complex; galectin-9; keratin; ciliary rootlet coiled-coil protein; kinesin-like protein KIF17; plectin 1 (intermediate filament binding protein); plastin-2 |
| Vesicular trafficking (ARF, Rab/Rho GTPases) | ADP-ribosylation factor (ARF) 1/3/4/5; ARF GTPase-activating protein GIT2; Rab 5/7/11/27; Rap1; Rho/Rac GEF 2; cell division cycle 42 (CDC42); transferrin-receptor protein 1; clathrin heavy chain; diaphanous homolog (mDia) 1 | ADP-ribosylation factor (ARF) 1/3/4/5; ARF GTPase-activating protein GIT2; Rab 1/5/7/8/10/11/35; Rho-associated protein kinase 2; Rap1; Rho/Rac GEF 2; cell division cycle 42 (CDC42); STE20-like kinase; citron (Rho-interacting, ser/thr kinase 21); integrin-linked kinase; transferrin-receptor protein; clathrin heavy chain; mDia 1 |
| Signal transduction (protein kinases and phosphatases) | Protein tyrosine kinase (Fyn/Lck); Ser/Thr-protein kinase PAK; P21-activated kinase (PAK) 2; casein kinase 2; sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA); fibroblast growth factor receptor (FGFR) 2; histone deacetylase 1/2 | Protein tyrosine kinase (Fyn/Lck); Ser/Thr-protein kinase PAK; P21-activated kinase PAK2; casein kinase 2; sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA); Ca2+/calmodulin-dependent protein kinase; fibroblast growth factor receptor (FGFR) 2 |
| Immune response | MHC class I antigen; Fc fragment of IgE gamma | MHC class I antigen; Fc fragment of IgE gamma |
| Metabolism | Enolase; adenosine kinase; phosphofructokinase; pantothenate kinase (CoA Biosynthesis); cytochrome b5 reductase; cytochrome c oxidase; NAD(P) dependent steroid dehydrogenase | Phosphofructokinase; adenosine kinase; pyridoxal (vitamin B6) kinase; pyruvate kinase; deoxycytidine kinase; NADH dehydrogenase; cytochrome b5 reductase; cytochrome c oxidase; manganese-superoxide dismutase (SOD) |
| Cell-cycle regulation | Cyclin-dependent kinase 20 | Cyclin-dependent kinase 20; apoptosis inhibitor 5 |
1Average abundance of human proteins was determined by comparing the LC–MS peptide peak intensity information of the same peptides from infected HL-60 cells to that from uninfected cells. Proteins with ratios greater than 2 (816 A. phagocytophilum and 1053 E. chaffeensis proteins) were identified and summarized in Tables S10 and S11 in Supplementary Material. Only proteins relevant to critical pathways affected by bacterial infection or with functions characterized previously were listed in this summary table.