Figure 2.

EGFP knockdown efficacy in vitro in transfected MiaPaca II cells using lipofectamin 2000. The siRNA molucules and nomenclature used are as depicted in Figure 1. Three concentrations were used: 1 nM, 5 nM and 25 nM. Top, mRNA levels were measured 24 hrs post transfection, and were corrected for 28S ribosomal RNA levels. Botom, Protein levels were measured 72 hrs post transfection and are corrected for eEF2α expression. MM indicates the control mismatch siRNA.