Figure 3.

Kinetics of PNA1 binding to three target sequences containing a single mismatch. The PvuII-PvuII fragment of plasmid pSM-1, pSM-2 or pSM-3 (312 bp) was incubated at 42°C with γ-PNA in the presence of 35 mM Na⁺ at various times. The PNA concentration was 1.5 µM and the dsDNA concentration was 10 nM. (A) Plots of fraction of complexes as a function of time with targets SM-1 (squares), SM-2 (triangles) or SM-3 (circles). Inset: A representative kinetic experiment with SM-3. (B) Semilogarithmic plots of the kinetics of strand displacement reactions. The initial binding rates were obtained from the slopes of lines in plots of -C_e ln (1 - C/C_e) versus time t. C_e and C denote the yield of product at equilibrium and at time t, respectively.